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This is independent research funded by a Medical Research Council Clinical Research Training Fellowship (grant number MR/T028084/1 awarded to L.A.J.) and carried out at the National Institute for Health and Care Research (NIHR) Maudsley Biomedical Research Center (BRC)
The fit of the data reports a dissociation constant for NADPH of 20 1 M followed by hydride exchange between the flavin and NADPH with rate constants in both directions close to 1,000 s 1 to predict an observational rate constant of 2000 s 1 , broadly similar the conclusions drawn from the CCD data (Figure 5A)
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