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Dual wavelength for reading in ELISA assay mainly aims to remove non-specific background interference, and improve accuracy and reliability of the detection

Before the cell testing, we analyzed the pH of the samples tested and we excluded the pH of copper compounds as the influencing factor of their potential cytotoxicity (SI 2)

3 Extended Administration Pharmacokinetics in DIO Mice Neelakantan et al., Biochemical Pharmacology (2024) This study reported a 28-day once-daily administration protocol of 5-Amino-1MQ in diet-induced obese mice, characterizing plasma pharmacokinetics, tissue distribution, and effects on body composition, fatty liver pathology, and circulating biomarkers

GSH), a predominant non-protein thiol in mammalian cells, typically exists in concentrations of 0.5 to 10 mM

This supports the fact that higher enzyme expression level may lead to higher LC 50 values