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[8] The age-related decline and its implications The decline of circulating GHK from 200 ng/mL at age 20 to 80 ng/mL by age 60 correlates with reduced wound healing capacity, thinner skin, decreased collagen density, and slower tissue repair

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50 our findings suggest that sperm quality and cryotolerance in dogs remain relatively stable throughout much of their lifespan but decline more noticeably in individuals aged nine years and older

doi:10.1007/s12178-025-09990-7