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Embryos treated with NOx and those non-treated (the control, embryos of aged seeds) were placed in Petri dishes filled with moistened filter paper for 48 h in a growth chamber (Sanyo MLR-35OH) at 25/20 C day/night, a 12/12 h photoperiod, the light intensity of 100 mol PAR m 2 s 1
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RNA samples were treated with the TURBO DNA-free kit (Applied Biosystems, Foster City, CA), further purified through an RNease kit (Qiagen) and quantified with a NanoDrop-1000 (NanoDrop Technologies Inc., Wilmington, DE)
Yang, R
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