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Its mature protein is mainly produced by renal tubular epithelial cells and subsequently secreted into the bloodstream, which may become the first barrier involved in mediating redox balance (Zhao et al., 2019
However, it is also the most invasive and expensive option
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For comparison, a procedural blank (HPLC water which was treated to all the same handling procedures as the samples) is also included
This establishes baselines and identifies any changes that might warrant attention
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