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For this, 1 mL of ECL solution A (100 mM Tris-HCl pH 8.5, 2.5 mM luminol, 0.4 mM p -coumaric acid) and solution B (100 mM Tris-HCl pH 8.5, 0.02% (v/v) H 2 O 2 ) were mixed in a 1:1 ratio and evenly applied to the membrane
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