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Consequently, despite the clear message of landmark trials such as the Diabetes Control and Complications Trial and the UK Prospective Diabetes Study regarding the need for excellent glycemic control, diabetes remains the leading cause of blindness, renal failure, and amputations

Huang YB, Jiang L, Liu XQ, Wang X, Gao L, Zeng HX, et al

Pancreatic proglucagon gene expression is promoted upon fasting and hypoglycaemia induction and inhibited by insulin

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The Eurasian Journal of Medicine

Gibco), while murine Pan02 cells were maintained in RPMI-1640 medium (Gibco) with 10% FBS