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mRNA quantification by quantitative real-time PCR (qRT-PCR) Total RNA was isolated using QIAzol-Reagent (Qiagen, Germany), based on modified Chomczyski & Sacchis method (Chomczynski and Sacchi, 2006), following the manufacturers protocol

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In 2021 alone, GI-related healthcare expenditure was estimated at $111.8 billion, reflecting both clinical and economic challenges

Among these, EGCG has been reported to display neuroprotection through various molecular mechanisms

In addition to side effects of a rapid increase of glutathione, it can also have risks associated with injections in general, and in the quality and reliability of any other ingredients added to the cocktail

Khan 2014)