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Liraglutide, Semaglutide, and Tirzepatide are well-studied glucagon-like peptide 1 (GLP-1) agonists that may be used as an option for patients needing assistance with metabolic issues and improving body composition

Melanotan II is a synthetic peptide that mimics alpha-melanocyte-stimulating hormone (-MSH), used primarily for inducing tanning , and secondarily for increasing libido and reducing appetite

Transporter proteins such as ABCG2, OAT1, and OAT3 present on the epithelial cells of the renal proximal tubules serve as important outlets mediating urate excretion, while ABCC4, a known organic anion transporter protein, also plays a role in urate excretion, though its specific location remains unclear

Traditionally used in Chinese and Ayurvedic medicine for centuries, berberine has gained considerable attention in recent years as a dietary supplement for metabolic health, particularly in relation to blood glucose regulation and weight management

For the second staining, the primary antibodies were: rabbitized GLP-1R antibody (0.12 g/ml), mouse GLP-1 antibody (0.5 g/ml), and a goat antiserum against ChAT (Millipore, Cat# AB144P

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