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doi: 10.1038/cddis.2012.47 328 DaiZCaiWHuFLanYLiLChungCet al

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After 96 hours, 10 l of the PrestoBlue reagent (A13261, Invitrogen) was added to each well, and plates were incubated at 37 C for 20 minutes

Interestingly, phosphocreatine was not detected in the plasma of both Phase-I and Phase II HSB (Table 1), indicating that the muscle and other tissues of the fish do not release phosphocreatine

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