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In contrast to ciprofloxacin-treated cells, we observed typical kanamycin-treated cells to lyse immediately after hypoosmotic shock (Supplementary Movie 10), as would be consistent with a scenario in which kanamycin-induced membrane damage is more widespreadpotentially due to ribosome-independent ionic interactions with the outer membrane 20,26 and results in a lower resistance of the membranes to stretching
In particular, we observed previously unreported, robust suppression of p70S6K, as well as suppression of p-4E-BP1 and p-S6 (Fig
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Cardiovasc Eng Technol 7:4457
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Exact dosing varies by experimental design and species but generally follows preclinical trial standards for NNMT inhibitors
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