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1.2 10 5 SHSY-5Y cells were subjected to similar treatment as above except 1 mM MPP + (for 24 h) was used to induce ROS

doi: 10.1038/nature07039

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It has a molecular weight of approximately 1419.53 Da and carries a net negative charge at physiological pH

The argument is not that a single early appointment is a strain, but that the precedent of making such exceptions could be