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The rate limiting step in the production of melatonin is arylalkylamine N -acetyltransferase, which has also been called the timezyme. The chart to the left shows how L-tryptophan is converted to 5-hydroxy tryptophan which is converted to serotonin and ultimately to melatonin

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334 This process leads to the induction of T-cell anergy, cell death, the differentiation of CD4+ cells into Tregs and polarization of macrophages to acquire an immunosuppressive phenotype

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Since it contains naturally occurring human chemicals, it carries minimal side effect risks

In rodent work, subcutaneous administration is the route most commonly used for both compounds in published mechanistic studies