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SDSPAGE and western blotting Samples were fractioned by SDSPAGE on 8% gels under reducing conditions, immunoblotted onto nitrocellulose membranes (GE Healthcare) and bands detected by enhanced chemiluminescence (GE Healthcare) onto films developed on a Xograph Compact X5 processor
Glutathione Biology: Primary cellular antioxidant made from cysteine, glycine, and glutamate
Antioxidants ., 14 , 617 (2025)
Libraries were prepared using the Universal Plus mRNA-seq protocol (Tecan) according to the manufacturers protocol
Zinc import is thought to involve the Ca-activated Mg-ATP carrier SLC25A25 (SCaMC-2), which can transport zinc into the matrix in addition to its canonical substrates 91
This inflammatory burden contributes not only to insulin resistance but also to pain sensitization
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