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JC-1 probe, Calcein-AM/PI Cell Viability/Cytotoxicity Assay Kit, ROS Assay Kit, and Cell lysate was purchased from Beyotime (China)

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The quantification of lipid peroxidation was performed by measuring the nmol MDA/gm wet tissue using the extension coefficient of MDA (32.54) of the method outlined by OhkawaOhkawa, et al