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Pull the chosen diluent volume into a sterile transfer syringe

This was implemented in HOOMD by using a DPD conservative pair force between all pair interactions involving the bulk PEG particles, with the force coefficient A = 25.0 and a cut-off distance of 2 1/6 ij , where ij is the distance between particle pairs i and j

Cell pellets were resuspended in reducing lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 10 mM DTT, 0.01 mg ml 1 DNase I) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator

They considered that previous results concerning the effect of GH on mitochondrial biogenesis were based on the incorrect interpretations of the data, as increased respiration does not necessarily reflect increased mitochondrial biogenesis

Sandstrom, J., Carlsson, L., Marklund, S.L

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