The extra waiting time preserves peptide integrity that rushing destroys
Although ICH Q2(R2) is primarily intended for pharmaceutical analytical procedures, its fit-for-purpose validation principles may be used as a non-mandatory scientific reference for research-reagent testing
A perfectly timed dose of degraded peptide achieves nothing
Moreover, recent advances have revealed that berberine and its derivatives promote the elimination of neurotoxic misfolded proteins, which can be used as a potential treatment for neurodegenerative diseases (Rusmini et al., 2020)
Townsend DM, Manevich Y, He L et al (2008) Novel role for glutathione S-transferase
Given that GHK-Cu is a naturally occurring molecule present in human plasma, concerns about long-term topical use seem largely theoretical