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Membranes were blocked with 5% non-fat milk and incubated with mouse anti-5mC (#61480, Active motif) or anti-5hmC (#39999, Active motif) followed by a horseradish peroxidase-conjugated antibody (Santa Cruz Biotechnology)

10.1016/j.earlhumdev.2022.105588 25 BraunschweigD.Van De WaterJ

Research suggests this may be due to higher concentrations of beta-3 adrenergic receptors in these fat depots

Pick one location and stay with it, because moving a vial in and out of the fridge creates condensation on the stopper, and a wet stopper is harder to disinfect properly

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This is important context for anyone building protocols around this compound