NJWA and MS performed phosphoproteomic analysis
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W., Pollock, C
The cells were scraped, spin down, and resuspended in HEPES buffer (100 mM HEPES, pH 7.6, 15 mM magnesium acetate and 10 mM DTT) with freshly added protease inhibitor (Roche Applied Science)
Clinical studies and meta-analyses suggest that ALA supplementation can lead to small but meaningful improvements in fasting glucose and insulin sensitivity, particularly in people with type 2 diabetes or metabolic syndrome.2 24 That said, these effects are generally modest and ALA should never be viewed as a substitute for prescribed diabetes medications
1, 2, 4, and 5 the replicate experiments are shown in Supplementary figures, as indicated in the figure legends