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This is standard practice
GHK-Cu chelates Cu and modulates collagen, decorin, and matrix-metalloproteinase gene expression in dermal fibroblast cultures
Aging involves cumulative oxidative damage to cells, proteins, and DNA
Cells treated with test compounds were washed with PBS and incubated with 10 M 2,7-dichlorofluorescein diacetate (DCFH-DA) at 37 C for 30 min, followed by washing with PBS, digestion with trypsin, and collection
doi: 10.1016/j.addr.2014.05.010 139 JiangQLiuCLiCPXuSSYaoMDGeHMet al