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General cell biology methods RPMI-1640 medium, DMEM, Iscoves modified Dulbeccos medium (IMDM), FBS, penicillinstreptomycin (10,000 U per ml sodium penicillin G and 10,000 g ml 1 streptomycin), trypsinEDTA solution (1) and PBS (1) were purchased from Gibco (Invitrogen)

Subsequently, membranes were probed at 4 C overnight with specific primary antibodies: RhoA (Mouse monoclonal 1:800 Millipore, Cat#05-778, Lot#3064837)

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doi: 10.1152/physrev.00039.2005 61 PengYJNanduriJRaghuramanGSouvannakittiDGadallaMMKumarGKet al

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