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The total flushing volume should not be too large to prevent microbial damage

Read the mass from the vial and the batch paperwork, not from a listing title, and record the volume you actually added rather than the volume you intended to add

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Briefly, 3 mL of TBA reagent was added to 1 mL of tissue homogenate, followed by vigorous agitation and then for 15 min at room temperature incubate the mixture [29]

Timeline and What to Expect Setting realistic expectations helps maintain consistency through the gradual improvement process that micro-dosing protocols typically produce

If it warmed above that, treat it as room temperature exposure and subtract those hours from your 21-day allowance