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Cycling strategies vary by peptide and by the overall goals of the protocol

Digested samples were desalted using a C18 spin column (Pierce C18 Spin, #89873) according to manufacturer instructions prior to resuspension in EPPS buffer and CH 3 CN for TMT labeling as described above

Example schedule only

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The animals were divided into three main groups defined as follows: MCAO + Vehicle included animals subjected to MCAO and treated with vehicle (saline)