Experimental data have already demonstrated that over 70 percent of the expressed proteins in spirulina remained intact after two hours of incubation under simulated gastric conditions that would fully degrade the same protein in a purified form within minutes
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The protein concentration of the resulting lysates was determined using the Quick Start Bradford Protein Assay Kit 2 (#5000202, Bio-Rad, USA)
Levels of Jasmonic acid (JA) were measured by liquid chromatographymass spectrometry (LCMS) with a mobile phase of 0.1% formic acid and acetonitrile, a flow rate of 0.3 mL/min, an injection volume of 5 l, and a column temperature of 30 C
Discussion The original method faced several challenges, including the need for strong acids to terminate the enzymatic reaction, which could be hazardous and affect the accuracy of the measurement
Osteopenia, a condition characterized by low bone density, can eventually progress to osteoporosis 2