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For actin and Drebrin immunoblots, proteins were prepared in NuPAGE LDS sample buffer (4) (Life Technologies, NP0007), split into reduced (+2% 2-ME) and nonreduced aliquots and heated at 70 C for 10 min 28
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Not as commonly used as plain bacteriostatic water but acceptable for most peptides
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Symptoms of vitamin B12 deficiency include
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