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The membrane was blocked with PBS containing 0.5% tween (PBST) and 5% milk, and probed overnight with affinity-purified anti-GspD or anti-PilQ in PBST plus 3% BSA

Continued treatment is necessary to maintain the skin tone

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But six months is long enough to notice, and it points the same direction the research does

The regimen was generally well tolerated, with manageable toxicities, and demonstrated expected pharmacodynamic effects consistent with metabolic modulation

AZ: Funding acquisition, provided resources and edited the manuscript