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Water: Absolutely fine

Johnston MV, Blue ME, Naidu S

To further characterize compound engagement of TGR in ex vivo worms using an orthogonal assay, inhibition of TGR activity in newly transformed schistosomula (NTS, skin-stage worms) was assessed using a TrxR-selective fluorescent probe 43,44 , TRFS-Green

3G, H) after HT treatment for 48 h

Agar gels were diluted with 1 mL PBS, melted at 85 C for 3 min, and centrifuged at 40 C (5 min at 1800 g ) to pellet the cells

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