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It ensures the testing results are objective and reliable for research use

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Melatonin has a central role in regulating circadian biological processes, most recognizably the sleep cycle

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Behavioral measurements should primarily include the percentage of time spent in the open quadrant, the percentage of entries into the open quadrant, the number of times the animal peeks down from the edge of the platform while in the open or closed quadrant, and the number of stretch-attend postures from the closed quadrant to the open quadrant