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Extraction and cultivation of primary rat NPCs Ten SD rats (8 weeks old, male) were sacrificed with an overdose of 2% (w/v) pentobarbital sodium

Slightly higher doses of CCK-8 administered via intraperitoneal injection (~110 g/kg) activate vagal afferents and increase neuronal activation in the NTS where VANs terminate, while vagotomy and capsaicin treatment abolish the suppressive effects of CCK-8 administration 177,178

Results: The number of human genes associated with stem cell function was 57 genes in the range of increases of 50% UP and 46 genes in the range of decreases of 50% DOWN

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