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The However, these amounts are general estimations

Measurement: Measure the optical density (OD) at 450 nm using a microplate reader immediately (within 15 minutes of adding the stop solution)

After quantifying the concentrations of melatonin, serotonin, dopamine, glutamate, and neurotrophic factors using HPLC and ELISA, the results are analyzed using statistical software such as GraphPad Prism

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