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(2) Bioavailability challenges : Many active ingredients of TCM exhibited suboptimal pharmacokinetic profiles due to structural instability, rapid oxidation, and poor membrane permeability, advanced delivery systems (e.g., lipid-based encapsulation, nanoparticle carriers) can be considered

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For full-length COP1 , it was constructed as a N-terminal Glutathione S-Transferase (GST) tag followed by a TEV cutting site