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Ice crystals physically disrupt the peptide structure, rendering it biologically inactive

2, 21 Thick frozen tissue sections (20 m) were incubated for 2 h at room temperature in the incubation solution containing 170 mM Tris-HCl buffer (pH 8.2), 1% bovine serum albumin (BSA), 40 g/ml bacitracin, 10 mM MgCl 2 , and 15 000 c.p.m./100 l 125 I-GLP1(736)amide (2000 Ci/mmol

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More robust trials are needed to determine who may benefit, which medicine works best, and what the long-term mental health effects may be