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6,8 Studies looking at higher doses, such as one comparing 12.5mg, 25mg, and 50mg, found a non-dose-dependent steady-state level maximum at the 25mg dose

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The BIOM file output from the DADA2 pipeline and the DECIPHER-aligned FastTree phylogenetic tree were input into QIIME (v1.9.1) and the scripts in the Supplementary Materials (QIIME Scripts compilation) were used to establish the alpha diversity metrics of Chao1 richness estimate, Simpsons evenness measure E, Shannon diversity index H, Simpsons diversity index, and Faiths phylogenetic diversity metric (whole tree) as well as the beta diversity measures of unweighted and weighted UniFrac analyses (the latter of which factors in ASV abundance 37 ) and BrayCurtis dissimilarity analysis

The analysis covers the cellular mechanisms of intrinsic factor synthesis, its interaction with vitamin B12, and the complex absorption process that occurs in the terminal ileum