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Extracts were cleared by centrifugation at 15,000 g for 15 min at 4 C, and supernatants were incubated with 2 g of purified GST-tagged GFP-Nanobody for 30 min on ice

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One unit of J3420 equals up to 1000 mcg

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And no matter what time you go to bed, make sure you invest in a cheap cotton eye mask, just a full organic cotton eye mask that you can put over your eyes and block out all the light