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390 However, the mechanisms by which fMLP suppresses the PI3K pathway via p38 MAPK remain elusive, as does the reason behind the co-stimulation of chemokines and fMLP leading to PTEN redistribution across the cell membrane
Two publications in 2000 mapped the biochemistry
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Lysates were boiled at 95 C for 5 min, 20 g of total protein were subjected to separation by a 10% SDS gel, transferred onto nitrocellulose membranes (Amersham Biosciences) and blocked with 5% milk powder in PBS Tween (0.1%) for 1 h
