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many patients are getting prescriptions from primary care doctors rather than weight-management specialists

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The next day, samples were incubated with Alexa Fluor 488-conjugated anti-IgG secondary antibodies and Alexa Fluor 647-conjugated anti-IgG for 1 h and stained with DAPI for 7 min

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After 24 h culture, the transfected cells were seeded into 384-well microtiter plates at a density of 3,000 cells per well in HBSS supplemented with 5 mM HEPES, 0.1% (w/v) bovine serum albumin (BSA) and 0.5 mM 3-isobutyl-1-methylxanthine