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cDNA was subsequently used to assess mRNA expression by qRT-PCR (QuantStudio 5 System, Thermo Fisher Scientific) with TaqMan Fast Advanced Master Mix (Thermo Fisher Scientific, 4444557) and TaqMan Gene Expression Assays (Thermo Fisher Scientific)
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400 mg daily) was more effective than black cohosh alone (both 13 mg and high dose of 1,000 mg daily) or placebo for alleviating menopausal symptoms, particularly mood symptoms, and increasing quality of life
Biol 95 MoldoveanuT.FollisA
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