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TGX Precast Gel (BioRad) was used to separate proteins within samples at 200V for 30min
doi: 10.1016/j.biopha.2023.115772 236 WangXLiuZPengPGongZHuangJPengH
Samples were recorded at 25 C (three 30-second recordings per specimen) and processed using NTA v3.2
It has been shown that, using model peptides, Sirt6 efficiently removes long-chain acyl groups 27,28,29 , and that Sirt4 removes acidic acyl groups, such as methylglutaryl, hydroxymethylglutaryl, and 3-methylglutaconyl ones 30
What doses are used in human studies for each peptide
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