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Ratiometric image analysis was conducted using a Python script (available from The background was subtracted for each channel and the ROIs (=transfected cells) were determined by thresholding or with the Cellpose 40 algorithm in the channel of the FP

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In light of the concerns regarding contamination and impurities, numerous other manufacturers of hand sanitizers were issued warning letters with citations for failure to adequately test components or finished drug product to ensure they met standards for identity, strength, and purity