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Correct insertion yields a novel NcoI site generated by ligation of the blunted ends, and so NcoI (NEB) linearization of the supercoiled vector was used to determine correct insertion (Figure 1b, lower panel)

Like any medical procedure, however, there are inherent risks involved

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Speaker: Dr Paola Pellegrini (XJTLU - Xi'an Jiaotong-Liverpool University) 89 Performance Analysis of 15-Minute City Zones Through Spatial and Machine Learning Techniques With the significant growth of urban populations, cities are expanding rapidly, making urban sprawl a critical global challenge that necessitates urgent attention in policy agendas

Statistical analyses Statistics were performed using SigmaStat Statistical Analysis software (SPSS Inc., Chicago, IL)