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After thawing, the samples were diluted 1:2 with chromatography water followed by sonication (25 min, 20C, SONOREX RK 100, Bandelin, Berlin, Germany) and centrifugation (21,000 x g, 10 min, 4C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany)
Metabolic changes included a reduction of the usage of muscle glycogen (i.e., its carbohydrate stores) during low-intensity exercise (consistent with an increase in lipid utilization) and streamlined glucose metabolism and mitochondrial flux during high-intensity exercise, leading to reduced anaerobic ATP production (which is what leads to lactic acid build-up in muscles)
These effects contribute to better blood sugar control and weight loss
Quercetin potentializes the respective cytotoxic activity of gemcitabine or doxorubicin on 3D culture of AsPC-1 or HepG2 cells, through the inhibition of HIF-1 and MDR1
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