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The scRNA/CITE-seq libraries were prepared using the GemCode Single Cell 3 Gel Bead and Library kit, version 3 (10x Genomics 1000128) according to the manufacturers instructions with the addition of amplification primer (3 nM, 5CCTTGGCACCCGAGAATT*C*C) during cDNA amplification to enrich the TotalSeq-A cell surface protein oligos
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Diabetes 61(6):15731583 Suzuki T, Elias BC, Seth A, Shen L, Turner JR, Giorgianni F et al (2009) PKC regulates occludin phosphorylation and epithelial tight junction integrity
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Reducing necessary read depth by adding a pull-down step Moreover, to extend the analysis to larger scales and increase the sensitivity, we added a streptavidin-based pull-down step to enrich the modified RNAs and increase the RT deletion rate (Supplementary Note, Supplementary Fig
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