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Serial dilutions of unfused albumin variants (0.034375.2 nM) or GLP-1 albumin fusions (0.031368.5 nM) (100 L/well), diluted in PBS/T/M (pH 5.5 or pH 7.4), were added for 1 h at room temperature

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Rotate injection sites within the same region in order to reduce the risk of cutaneous amyloidosis

The markings are designed for insulin, but they work perfectly for measuring reconstituted peptides