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In the RT structure, the cyano group at the position of the Michael acceptor enables fast reaction kinetics, the four-membered azetidine ring improves quantum yield and photostability 17 , and the two carboxylic acid groups ensure aqueous solubility and reduce probe binding to hydrophobic cellular structures

Glass bottom dishes were used for cell culture due to confocal scanning requirements

This is a key paper delineating the cell types that compose fibroids

Immunoprecipitation Hepatocytes were lysed in an immunoprecipitation (IP) buffer (150 mM NaCl), 50 mM Tris-HCl, 1% Nonidet P-40, pH 7.8, and a mammalian cell-specific protease inhibitor cocktail

Higher, clinically studied doses (500mg or more) tend to show results more reliably