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Once the cell suspension is completely thawed, add Stimulation Buffer (Hanks balanced salt solution (HBSS) supplemented with 5 mM HEPES, 0.5 mM IBMX and 0.1% (w/v) BSA, pH 7.4) and centrifuge at 1000 rpm for 5 minutes

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Frontiers in Aging Neuroscience, 14 , 888292

or 2) basal insulin metformin for at least 90 days were enrolled in this randomized, doubleblind study