temperature gradient, 70C (1 min), ramp to 230C (15C/min, 2 min hold), ramp to 325C (25C/min, 3 min hold)
Blood Cancer , 63 , 1349-1356, doi: Tagde, A., Singh, H., Kang, M
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Blots were blocked with phosphate buffered saline with 0.1% Tween 20 and then binding with antibodies for eNOS (1: 1000) or caveolin-1 (1: 1000), followed by horseradish peroxidase-conjugated goat anti-rabbit (diluted 1: 5000) and enhanced chemiluminescence reaction was performed for blots development