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Common requirements include documented BMI meeting the threshold, proof of previous weight loss attempts (usually 3-6 months of supervised diet and exercise), at least one qualifying comorbidity, and sometimes evidence that other treatments have been tried and failed

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the expected molecular mass

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Initial investigations may include complete blood count, metabolic panel, thyroid function tests, hemoglobin A1c, ESR/CRP, HIV testing, and urinalysis when clinically indicated

Furthermore, this reduced binding might also affect the manner in which IGF-1 LR3 interacts with targeted tissues within experimental frameworks