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The accumulation of senescent cells, often referred to as the "old cell burden," is a concern because these cells can secrete a variety of harmful substances as part of the senescence-associated secretory phenotype (SASP)

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Ichimura Y, Habuchi T, Tsuchiya N, Wang L, Oyama C, Sato K, et al

Then, divide the dose you need by the concentration to get the volume

When evaluated after exposure to LASSS at a 1:8000 molar ratio to HGF and subsequent ultra-filtration to wash-out un-reacted free LASSS, c-met binding affinity increased more than two-fold over the original non-nitrated HGF

The study selected 2.5 mM DTNB for further experiments