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2a), with the exception of the extracellular loop (ECL) 1 and intracellular loop (ICL) 3, which were not modelled

Alternative approaches are typically recommended in these cases

Research suggests VIP has neuroprotective and neurotrophic properties, supporting bloodbrain barrier integrity, reducing oxidative stress, and modulating neuroinflammation, with relevance in neurodegenerative models such as Alzheimers and Parkinsons disease

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This step helps reduce the risk of getting bacteria in the injection site