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Interestingly, the phosphodiesterase inhibitor Cilostazol (used in peripheral vascular disease treatment) is able to improve cognitive function in mice by increasing the hippocampal production of IGF-I through stimulation of sensory neurons[350]

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This process is called endogenous synthesis, and it primarily takes place in the liver, though almost every cell has the equipment to do it

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The membrane was treated with ECL prime blocking solutions (GE Healthcare, Little Chalfont, UK) and reacted overnight at 4 C with each antibody (Table S1) diluted in blocking buffer

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